The identification and characterization of a novel protein that interacts with the catalytic subunit of Arabidopsis thaliana protein phosphatase 2A
Dublin Core
Title
The identification and characterization of a novel protein that interacts with the catalytic subunit of Arabidopsis thaliana protein phosphatase 2A
Subject
Arabidopsis thaliana -- Genetics
Phosphoprotein phosphatases
Creator
Harris, Darby McGrady
Date
2005
Contributor
Rundle, Sabine J.
Rights
http://rightsstatements.org/vocab/CNE/1.0/
Format
application/pdf
manuscripts (documents)
Type
Text
Identifier
61763
https://southernappalachiandigitalcollections.org/object/61763
Access Rights
Limited to on-campus users
Abstract
Type 2A serine/threonine protein phosphatases (PP2A) have been implicated as important mediators of a number of plant growth and development processes. In an effort to identify novel substrates and/or regulators of plant PP2A, a yeast two-hybrid screen of an Arabidopsis cDNA library was performed using the catalytic subunit of Arabidopsis PP2A (PP2Ac) as bait. All true positives identified from this screen were derived from the same gene, which has been named TAP46 (2A phosphatase Associated Protein of 46 kDa). Further yeast two-hybrid experiments showed that the TAP46 protein does not appear to require the A regulatory subunit of PP2A for binding to PP2Ac, and is therefore a novel plant PP2A-binding protein. Immunoprecipitation assays using antibodies generated to a peptide spanning amino acids 356 to 366 of TAP46, indicated that TAP46 is associated with a type 2A protein phosphatase in plant cells. A search of the database with the protein sequence of TAP46 indicated that its most significant match was to that of a chilling-induced protein from rice with 43% identity and 59% similarity. The next two most significant matches were the mammalian protein a4 and the Saccharomyces cerevisiae protein TAP42 at 42% and 38% similarity, respectively. Both TAP42 and a4 are known homologs of each other and both have been shown to associate directly with PP2Ac and other related phosphatases. In addition, TAP42 and a4 have been shown to function in the target-of-rapamycin (TOR) signaling pathway, which connects nutrient availability with protein synthesis. The results presented in this thesis show that TAP46 is a novel plant PP2A-associated protein, add to the evidence of a TOR pathway in Arabidopsis, and also suggest a role for PP2A in this pathway
Date Created
2014-04-28
Rights Holder
All rights reserved. For permissions, contact Hunter Library Digital Collections, Western Carolina U, Cullowhee, NC 28723
Extent
2078 KB(file size)
x, 65 pages(pages)
Is Part Of
Western Carolina University Restricted Electronic Theses and Dissertations
Citation
Harris, Darby McGrady, “The identification and characterization of a novel protein that interacts with the catalytic subunit of Arabidopsis thaliana protein phosphatase 2A,” OAI, accessed June 8, 2025, https://sadc.qi-cms.com/omeka/items/show/61763.