The identification and characterization of a novel protein that interacts with the catalytic subunit of Arabidopsis thaliana protein phosphatase 2A

Dublin Core

Title

The identification and characterization of a novel protein that interacts with the catalytic subunit of Arabidopsis thaliana protein phosphatase 2A

Subject

Arabidopsis thaliana -- Genetics
Phosphoprotein phosphatases

Creator

Harris, Darby McGrady

Date

2005

Contributor

Rundle, Sabine J.

Rights

http://rightsstatements.org/vocab/CNE/1.0/

Format

application/pdf
manuscripts (documents)

Type

Text

Identifier

61763
https://southernappalachiandigitalcollections.org/object/61763

Access Rights

Limited to on-campus users

Abstract

Type 2A serine/threonine protein phosphatases (PP2A) have been implicated as important mediators of a number of plant growth and development processes. In an effort to identify novel substrates and/or regulators of plant PP2A, a yeast two-hybrid screen of an Arabidopsis cDNA library was performed using the catalytic subunit of Arabidopsis PP2A (PP2Ac) as bait. All true positives identified from this screen were derived from the same gene, which has been named TAP46 (2A phosphatase Associated Protein of 46 kDa). Further yeast two-hybrid experiments showed that the TAP46 protein does not appear to require the A regulatory subunit of PP2A for binding to PP2Ac, and is therefore a novel plant PP2A-binding protein. Immunoprecipitation assays using antibodies generated to a peptide spanning amino acids 356 to 366 of TAP46, indicated that TAP46 is associated with a type 2A protein phosphatase in plant cells. A search of the database with the protein sequence of TAP46 indicated that its most significant match was to that of a chilling-induced protein from rice with 43% identity and 59% similarity. The next two most significant matches were the mammalian protein a4 and the Saccharomyces cerevisiae protein TAP42 at 42% and 38% similarity, respectively. Both TAP42 and a4 are known homologs of each other and both have been shown to associate directly with PP2Ac and other related phosphatases. In addition, TAP42 and a4 have been shown to function in the target-of-rapamycin (TOR) signaling pathway, which connects nutrient availability with protein synthesis. The results presented in this thesis show that TAP46 is a novel plant PP2A-associated protein, add to the evidence of a TOR pathway in Arabidopsis, and also suggest a role for PP2A in this pathway

Date Created

2014-04-28

Rights Holder

All rights reserved. For permissions, contact Hunter Library Digital Collections, Western Carolina U, Cullowhee, NC 28723

Extent

2078 KB(file size)
x, 65 pages(pages)

Is Part Of

Western Carolina University Restricted Electronic Theses and Dissertations

Citation

Harris, Darby McGrady, “The identification and characterization of a novel protein that interacts with the catalytic subunit of Arabidopsis thaliana protein phosphatase 2A,” OAI, accessed June 8, 2025, https://sadc.qi-cms.com/omeka/items/show/61763.