Proteomic investigation of Pteris vittata (Chinese brake fern)

Proteomic investigation of Pteris vittata (Chinese brake fern)

Ferns -- Molecular aspects

Gel electrophoresis

Phytoremediation

Proteins -- Analysis

Pteris -- Molecular aspects

Liebschutz, Joshua Aaron

2005

Bonds, Wesley

Butcher, David J.

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Protocols were developed for performing 2-dimensional gel electrophoresis on proteins of Pteris vittata, an arsenic hyperaccumulator. This fern, a native of Asia, has naturalized itself to the southern U.S., and has become an object of interest to phytoremediologists. Generally standard protocols for plant protein 2-D electrophoresis were found sufficient, however further modification and analysis will be required to optimize experimental results. Standard gel staining techniques also seem suitable for use with this fern. Subsequent to optimization, the proteome of Pteris vittata can be analyzed to detect possible proteomic activity related to arsenic uptake. It is possible that understanding the molecular biology behind this fern's arsenic hyperaccumulation will enable technological advances in arsenic handling and remediation

2014-04-28

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